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Home > End-Modified Double-Strand DNA Donors for Clone-Free & Selection-Free Gene Knock-In in Mammalian Cells Lines

End-Modified Double-Strand DNA Donors for Clone-Free & Selection-Free Gene Knock-In in Mammalian Cells Lines

End-Modified Double-Strand DNA Donors for Clone-Free & Selection-Free Gene Knock-In in Mammalian Cells Lines [1]

Dr. Zhao from the University of IL has developed a rapid, cloning-free CRISPR/Cas9 based gene knock-in (KI) method that utilizes Cas9/gRNA ribonucleoprotein( Cas9 RNP) and end-modified double-strand DNA (dsDNA) generated by PCR. This method allows large gene (up to 1.1kb) knock-in with at least 5% KI efficiency in various mammalian cell lines. With high KI efficiency and short preparation time, this method can be applied in mammalian genome editing and engineering.
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[1] https://origin.otm.illinois.edu/technologies/end-modified-double-strand-dna-donors-clone-free-selection-free-gene-knock-mammalian